Are you centered? An automatic crystal-centering method for high-throughput macromolecular crystallography

Publication Type

Journal Article

Date Published

07/2007

Authors

DOI

Abstract

Crystal centering is a key step in macromolecular X-ray crystallography experiments. A new method using image-processing and machine-vision techniques allows the centering of small crystals in the X-ray beam. This method positions crystals even when the loop is initially out of the camera's field of view and adapts to the difficulty of the experiment. The process has been tested on many diverse crystals with a 93% success rate when compared with manual centering.

Journal

Journal of synchrotron radiation

Volume

14

Year of Publication

2007

Issue

Pt 4

Organization